Nomograph for the determination of the relative centrifugal fi eld for a given rotor
speed and radius. The three columns represent the radial distance (in mm), the relative
centrifugal fi eld and the rotor speed rpm (in min-1).

TLS Online TPP Program

#Id: 2518

#Unit 13. Methods in Biology

Electrophoresis of normal RNA can be done in a 2% agarose gel in about 1 h while, Ribosomal RNAs (18 S and 28 S) are clearly resolved in 2.5–5% acrylamide gradient gel with an overnight run. 

TLS Online TPP Program

#Id: 2519

#Unit 13. Methods in Biology

Capillary zone electrophoresis is the example of free solution electrophoresis with highest resolution using high voltage and using Electrophoretic mobility (EFM) & Electroendosmosis force (EEOF).

TLS Online TPP Program

#Id: 2520

#Unit 13. Methods in Biology

Capillary zone electrophoresis can analyze hybridization probes, gene-cloning, DNA sequencing, polymerase chain reaction (PCR), HIV load, site-directed mutagenesis, & antisense therapeutics.

TLS Online TPP Program

#Id: 2521

#Unit 13. Methods in Biology

Point mutations in DNA, quantification of DNA, Chiral compounds all ca be analyzed by using Capillary zone electrophoresis.

TLS Online TPP Program

#Id: 2522

#Unit 13. Methods in Biology

Output of capillary zone electrophoresis known as electropherogram.

TLS Online TPP Program

#Id: 2523

#Unit 13. Methods in Biology

Microchip electrophoresis is also based on free flow.