SeqA binds to hemimethylated DNA more strongly than to fully methylated DNA.

TLS Online TPP Program

#Id: 2792

#Unit 13. Methods in Biology

Chromatography columns are considered to consist of a number of adjacent zones in each of which there is sufficient space for an analyte to completely equilibrate between the two phases, Each zone is called a theoretical plate (having no N).

TLS Online TPP Program

#Id: 2793

#Unit 13. Methods in Biology

The length of column containing one theoretical plate is referred to as the plate height, H, which has units of length normally in micrometers.

TLS Online TPP Program

#Id: 2794

#Unit 13. Methods in Biology

Plate height is simply related to the width of the analyte peak, expressed in terms of its standard deviation s , and the distance it travelled within the column, x. Specifically

TLS Online TPP Program

#Id: 2795

#Unit 13. Methods in Biology

The smaller the value of H and the greater the value of N, the more efficient is the column in separating a mixture of analytes.

TLS Online TPP Program

#Id: 2796

#Unit 13. Methods in Biology

The number of theoretical plates in the whole column of length L is equal to L divided by the plate height:

TLS Online TPP Program

#Id: 2797

#Unit 13. Methods in Biology

Relationship between the number of theoretical plates (N) and the shape of the analyte peak.