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#Unit 3. Fundamental Processes
In mammalian systems (where the DNA polymerase does not have a 5'- 3' exonuclease
activity), Okazaki fragments are connected by a two-step process. Synthesis of an Okazaki fragment displaces the RNA primer of the preceding fragment in the form of a "flap’’.
The base of the flap is cleaved by the enzyme FEN1 (flap endonuclease 1 ) .