Oligomycin and Venturicidin, toxic antibiotics that bind to the ATP synthase are potent inhibitors of both ATP synthesis and the transfer of electrons through the chain of carriers to O2.

TLS Online TPP Program

#Id: 2999

#Unit 13. Methods in Biology

Flow chart of the fractionation protocol of these membranes from tissue homogenates
using differential centrifugation and density gradient methodology.

TLS Online TPP Program

#Id: 3000

#Unit 13. Methods in Biology

Crude differential centrifugation techniques can be conveniently employed to isolate
intact mitochondria and microsomes.

TLS Online TPP Program

#Id: 3001

#Unit 13. Methods in Biology

Caesium chloride is widely used for the banding of DNA and the isolation of
plasmids, nucleoproteins and viruses

TLS Online TPP Program

#Id: 3002

#Unit 13. Methods in Biology

Sodium bromide and sodium iodide are employed for the fractionation of lipoproteins
and the banding of DNA or RNA molecules, respectively.

TLS Online TPP Program

#Id: 3003

#Unit 13. Methods in Biology

For the separation of membrane vesicles derived from tissue homogenates, ultra-pure
DNase-, RNase and protease-free sucrose represents a suitable and widely employed
medium for the preparation of stable gradients.

TLS Online TPP Program

#Id: 3004

#Unit 13. Methods in Biology

For isopycnic separation , density centrifugation is continued until the buoyant density
of the particle of interest and the density of the gradient are equal.