The protein-coding region(s) of each mRNA is composed of a contiguous, non-overlapping string of codons called an open reading frame (commonly known as an ORF).

TLS Online TPP Program

#Id: 3130

#Unit 3. Fundamental Processes

Replication inserts the normal (nonmodified) bases into the daughter strands. This generates hemimethylated DNA, in which one strand is methylated and one strand is unmethylated.

TLS Online TPP Program

#Id: 3131

#Unit 3. Fundamental Processes

Initiation may require full methylation of the Dam target sites in the origin, or it may be inhibited by hemimethylation of these sites.

TLS Online TPP Program

#Id: 3132

#Unit 3. Fundamental Processes

The GATC sites at the origin remain hemimethylated for -13 minutes after replication.

TLS Online TPP Program

#Id: 3133

#Unit 3. Fundamental Processes

Initiation at oriC requires the sequential assembly of a large protein complex on the membrane.

TLS Online TPP Program

#Id: 3134

#Unit 3. Fundamental Processes

The ability of a plasmid relying upon oriC to replicate in dam- E. coli depends on its state  of methylation.

TLS Online TPP Program

#Id: 3159

#Unit 3. Fundamental Processes

 Eukaryotic cells have many DNA polymerases. The replication enzymes operate with high fidelity. Except  for the β enzyme, the repair enzymes all have low fidelity.