Nurturing Life Sciences
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#Id: 3038
#Unit 3. Fundamental Processes
Core polymerase and the clamp dissociate at completion of Okazaki fragment synthesis and reassociate at the beginning.
#Id: 3039
The length of the priming RNA is limited to eight to 14 bases. Apparently, DNA polymerase III is responsible for displacing the primase.
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#Id: 3041
Synthesis of an Okazaki fragment terminates just before the start of the RNA primer of the preceding fragment.
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FENl is an exo-jendonuclease that recognizes the structure created when one strand of DNA is displaced from a duplex as a "flap." In mammalian replication it cleaves at the base of the flap to remove the RNA primer.
#Id: 3043
The gap is filled by DNA polymerase I; pol A mutants fail to join their Okazaki fragments properly.