Chromosome has to be read under following heads

1. Packaging and packing proteins

2. Supercoiling

3. Loops, domains, centromere and telomere

4. Histones

TLS Online TPP Program

#Id: 2321

#Unit 3. Fundamental Processes

Splice sites are directional in nature. 5’ splice site -the left, or donor, site and the 3’ splice site - right, or acceptor, site.

TLS Online TPP Program

#Id: 2322

#Unit 3. Fundamental Processes

Splicing complexes

TLS Online TPP Program

#Id: 2323

#Unit 3. Fundamental Processes

Lariat formation is characterize by 2’-5’ phosphodiester bond

TLS Online TPP Program

#Id: 2324

#Unit 3. Fundamental Processes

Group II mitochondrial introns are excised by the same mechanism as nuclear pre-mRNAs via a lariat that is held together by a 2’–5’ bond.

TLS Online TPP Program

#Id: 2325

#Unit 3. Fundamental Processes

SR proteins are involved in exon definition in which they interact with U1 and U2AF forming commitment E complex.

 

TLS Online TPP Program

#Id: 2326

#Unit 3. Fundamental Processes

U11 base pairs with the 5’ splice sites; U12 base pairs with the branch point sequence near the 3’ splice site; and U4atac and U6atac provide analogous functions during the spliceosome assembly and activation of the spliceosome. 

U5 is same component in all splicing complexes