Mammalian H2ABbd variant is excluded from the inactive X and forms a less stable nucleosome than canonical H2A

TLS Online TPP Program

#Id: 804

#Unit 3. Fundamental Processes

RNA Polymerase SDS page shows two very large subunits: beta (b) and beta-prime (b’), with molecular masses of 150 and 160 kD, respectively.

TLS Online TPP Program

#Id: 805

#Unit 3. Fundamental Processes

The holoenzyme could transcribe intact phage T4 DNA in vitro quite actively, the core enzyme had little ability to do this.

TLS Online TPP Program

#Id: 806

#Unit 3. Fundamental Processes

Hinkle and Chamberlin experiment showed that the polymerase holoenzyme binds much more tightly to the T7 DNA than does the core enzyme.

TLS Online TPP Program

#Id: 807

#Unit 3. Fundamental Processes

Hinkle and Chamberlin experiment also showed that the melted DNA has higher affinity for holoenzyme

TLS Online TPP Program

#Id: 808

#Unit 3. Fundamental Processes

A promoter is defined by the presence of short consensus sequences at specific locations.

TLS Online TPP Program

#Id: 809

#Unit 3. Fundamental Processes

In the bacterial genome, the minimum length of promoter that could provide an adequate signal is 12 bp.  The minimum length required for unique recognition increases with the size of genome, a problem in eukaryotic genomes.