For subunit vaccine, a eukaryotic cloning host is always used because the recombinant proteins produced by bacteria are nonglycosylated, and glycosylation is necessary for the proteins to be immunologically active.

TLS Online TPP Program

#Id: 6688

#Unit 3. Fundamental Processes

The Nova and Fox families of RNA-binding proteins can promote or suppress splice site selection in a context-dependent fashion.

TLS Online TPP Program

#Id: 6689

#Unit 3. Fundamental Processes

Trans-splicing is rare in multicellular eukaryotes, it occurs as the primary mechanism to process precursor RNA into mature, translatable mRNAs in some organisms, such as trypanosomes and nematodes.

TLS Online TPP Program

#Id: 6690

#Unit 3. Fundamental Processes

 The SL RNA provides an exon that is connected to the first exon of an mRNA by trans-splicing. The reaction involves the same interactions as nuclear cis splicing but generates a Y-shaped RNA instead of a lariat.

TLS Online TPP Program

#Id: 6691

#Unit 3. Fundamental Processes

In C. elegans, about 70% of genes are processed by the trans-splicing mechanism

TLS Online TPP Program

#Id: 6692

#Unit 3. Fundamental Processes

A very different form of RNA editing is found in the RNA transcripts that encode proteins in the mitochondria of trypanosomes.

TLS Online TPP Program

#Id: 6693

#Unit 3. Fundamental Processes