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 The MTT assay can measure cell proliferation or cell death.

#Unit 13. Methods in Biology #Immunotechniques #Part B Pointers
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#Unit 13. Methods in Biology

The specificity of the PCR depends crucially upon the primers. The following factors are important in choosing effective primers


Primers should be 17 to 30 nucleotides in length


A GC content of about 50% is ideal. For primers with a low GC content, it is desirable to choose a long primer to avoid a low melting

 temperature.


Sequences with long runs (i.e., more than three or four) of a single nucleotide should be avoided.





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#Unit 4. Cell Communication and Cell Signaling

Heavy chain contain more constant region in compare to variable region, in contrast in the light chain variable and constant region are equal.

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#Unit 3. Fundamental Processes

Adenine is deaminated to hypoxanthine.

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#Unit 7. System Physiology – Animal

Hyperthyroidism Thyrotoxicosis

Graves’ Disease Toxic Goiter

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#Unit 3. Fundamental Processes

Prp5 is critical for U2 binding
Brr2 facilitates U1 and U4 release
Prp2 is responsible for the activation of the spliceosome Prp22 helps the release of the mature mRNA from the spliceosome

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