The total concentration of antibody in the equilibrium dialysis chamber is known, the equilibrium equation can be rewritten as:

TLS Online TPP Program

#Id: 2839

#Unit 13. Methods in Biology

These histidine's are separated by using Nickel-chelate-nitrilotriacetate (Ni-NTA) chromatography column.

TLS Online TPP Program

#Id: 2840

#Unit 13. Methods in Biology

These are His-tag subsequently removed by cleavage with a reagent such as cyanogen bromide and the protein of interest purified by protein biochemical methods such as chromatography and polyacrylamide gel electrophoresis.

TLS Online TPP Program

#Id: 2841

#Unit 13. Methods in Biology

Phosphorylated protein or amino acid are separated by using immobilized metal affinity chromatography ( IMAC), or immuno-affinity using antibodies against phosphorylated amino acids or peptides

TLS Online TPP Program

#Id: 2842

#Unit 13. Methods in Biology

GLUT separation is also done with the help of affinity chromatography, normally glut protein are cloned into the vector and then the help of affinity chromatography

TLS Online TPP Program

#Id: 2843

#Unit 13. Methods in Biology

Recovery of proteins using (6His-Tag) and (Ni-NTA) chromatography columns.

TLS Online TPP Program

#Id: 2844

#Unit 13. Methods in Biology

The glucose uniporter GLUT1 can be purified from the solubilized mixture by antibody affinity chromatography on a column containing either a GLUT1-specific monoclonal antibody or an antibody specific for the epitope tag, then incorporated into liposomes made of pure phospholipids.