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If the antibody preparation is polyclonal and has a range of affinities, Scatchard plot yields a curved line whose slope is constantly changing. The average affinity constant K0 can be calculated by determining the value of Ka when half of the binding sites are occupied (i.e., when r  1 in this example). In this graph, antiserum #3 has a higher affinity (K0  2.4  108) than antiserum #4 (K0  1.25  108)



#Unit 13. Methods in Biology #Immunotechniques #Part B Pointers
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#Unit 10. Ecological Principles

Loss of Biodiversity 
Direct ways: Deforestation, hunting, poaching, commercial exploitation.
Indirect ways: Loss or modification of the natural habitats, introduction of exotic species, pollution, etc.
Natural causes - Climate change



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#Id: 4518

#Unit 13. Methods in Biology

Waves are divided in various groups:

Alpha

Beta 

Delta  

Theta

Gamma 


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#Unit 11. Evolution and Behavior

Homo erectus– Invent fire

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#Id: 9514

#Unit 6. System Physiology – Plant

Quorum sensing in Erwinia carotovora is mediated by N‐acylhomoserine lactones, small molecules released from the bacterial cells into the surrounding medium.

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#Id: 7278

#Unit 6. System Physiology – Plant

Two chloroplast proteins ferredoxin–thioredoxin reductase and thioredoxin regulate five pivotal enzymes within minutes of the
light–dark transition:


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