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n. Carotenoids, for instance, are a large class of red, yellow and orange plant pigments composed of long carbon chains with many conjugated double bonds. They contain three maxima in the visible region of the electromagnetic spectrum (420 nm, 450 nm, 480 nm).

#Unit 13. Methods in Biology #UV/visible #Part B Pointers
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#Unit 13. Methods in Biology

FRET is possible only when the distance between donor and acceptor are within 1 to 10 Å

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#Unit 13. Methods in Biology

GFP chromophore comprises an oxidized form of the tripeptide –Ser65–Tyr66–Gly67–.

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#Unit 13. Methods in Biology

The efficiency of FRET is inversely proportional to the sixth power of the distance between donor and acceptor.

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#Unit 13. Methods in Biology

Short tandem repeats (STRs) are short repeated sequences of DNA (1–10 bp) that account for approximately  3% of the human genome.

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#Unit 13. Methods in Biology

AAAAAAAAAAAAAAA (a 1-base repeat) and CACACACACACACACACACACA (a 2-basen repeating), CAGCAGCAGCAGCAGCAG (a 3 base repeating) are most common.

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#Unit 13. Methods in Biology

Most STRs are found in the noncoding regions, while only about 8% locate in the coding regions.

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