The constructive method involved direct analysis of protein and gives absorbance at wavelength of 280 nm.

TLS Online TPP Program

#Id: 8916

#Unit 2. Cellular Organization

Cytokinesis

With the exception of higher plants, the division of the cell is brought about by a contractile ring made of actin and myosin

TLS Online TPP Program

#Id: 8917

#Unit 2. Cellular Organization

TLS Online TPP Program

#Id: 8918

#Unit 2. Cellular Organization

Centralspindlin is a heterotetramer composed of two copies of the kinesin-6 motor protein MKLP1 and a dimer of a Rho GAP

TLS Online TPP Program

#Id: 8919

#Unit 2. Cellular Organization

MKLP1 is inhibited by 14-3-3 to ensures that the spindle midzone microtubules do not compact until the end of anaphase

TLS Online TPP Program

#Id: 8920

#Unit 2. Cellular Organization

During anaphase, when cyclin B-CDK1 activity is extinguished but Aurora and Polo kinases are still active, Aurora B phosphorylates MKLP1 and displaces the 14-3-3, allowing compact the spindle midzone.

TLS Online TPP Program

#Id: 8921

#Unit 2. Cellular Organization

At the same time, Plk1 phosphorylates the Rho-GAP component, to form an active Centralspindlin-Ect2 complex.