The P2 cell is also critical in giving the signal that distinguishes ABp from its sister, ABa

TLS Online TPP Program

#Id: 2811

#Unit 13. Methods in Biology

In RPLC mobile phase is commonly water or aqueous buffers, methanol, acetonitrile or tetrahydrofuran, or mixtures of them.

TLS Online TPP Program

#Id: 2812

#Unit 13. Methods in Biology

 In RPLC the organic solvent is referred to as an organic modifier.

TLS Online TPP Program

#Id: 2813

#Unit 13. Methods in Biology

Reversed-phase liquid chromatography differs from most other forms of chromatography in that the stationary phase is essentially inert and only non-polar (hydrophobic) interactions are possible with analytes.

TLS Online TPP Program

#Id: 2814

#Unit 13. Methods in Biology

Proteins differ in the amount of hydrophobic amino acids that are present on their surface. This difference can be exploited in salt fractionation but can also be used in a higher resolution method using hydrophobic interaction chromatography (HIC).

TLS Online TPP Program

#Id: 2815

#Unit 13. Methods in Biology

HIC column is made up of phenyl-Sepharose, where phenyl groups are bonded to the insoluble support Sepharose.

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#Id: 2816

#Unit 13. Methods in Biology

When in the protein solution divalent salts, or multivalent salts the protein separated in form of aggregate.