Lineweaver-Burk plot showing the effect of competitive inhibition; (b) the same, showing plots for several inhibitor concentrations at fixed enzyme concentrations.

TLS Online TPP Program

#Id: 7427

#Unit 5. Developmental Biology

When the ratio of PSF relative to that of bacteria exceeds a certain threshold, cells stop proliferating and initiate the expression of genes that are required for their aggregation.

TLS Online TPP Program

#Id: 7428

#Unit 5. Developmental Biology

Early on during aggregation, gene expression requires the activity of cAMP-dependent protein kinase.

TLS Online TPP Program

#Id: 7429

#Unit 5. Developmental Biology

High PSF to food ratio is upregulate the translation of the PKA catalytic subunit PKA-C.

TLS Online TPP Program

#Id: 7430

#Unit 5. Developmental Biology

A second glycoprotein secreted during starvation is CMF (conditioned medium factor)

TLS Online TPP Program

#Id: 7431

#Unit 5. Developmental Biology

CMF stimulates gene expression in parallel with PSF, and both signals potentiate cAMP signalling by inducing genes involved in cAMP synthesis and detection

TLS Online TPP Program

#Id: 7432

#Unit 5. Developmental Biology

Newly activated genes are:

Adenylate cyclase A (ACA)

PKA

cAMP receptors (cARs)

Extracellular cAMP phosphodiesterase PdsA

An intracellular cAMP phosphodiesterase RegA