DnaA is an ATP-binding protein.

The first stage in initiation is binding of the DnaA-ATP protein complex to the fully methylated oriC sequence.

This takes place in association with the inner membrane. DnaA is in the active form only when bound to ATP.

DnaA has intrinsic ATPase activity that hydrolyzes ATP to ADP and thus inactivates itself when the initiation stage ends.

TLS Online TPP Program

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#Unit 2. Cellular Organization

Cot1/2 is concentration of ssDNA left after time t. T1/2 is time taken for half renaturation.

TLS Online TPP Program

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#Unit 2. Cellular Organization

Low Cot1/2 value means

1. Low complexity with high repeats

2. High complexity with high repeats

3. Low complexity with no repeats

4. no complexity and no repeats

TLS Online TPP Program

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#Unit 2. Cellular Organization

Number of Genes present in per million base pair of genome is called gene density.

TLS Online TPP Program

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#Unit 2. Cellular Organization

Escherichia coli K-12 is 4.6Mb genome with 4400 genes so gene density 950 genes/Mb.

TLS Online TPP Program

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#Unit 2. Cellular Organization

Homo sapiens has 3200 Mb genome in which 20,000 genes are present so Gene density will be 6.25 genes/Mb.

TLS Online TPP Program

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#Unit 2. Cellular Organization

Gene density has inverse relation with complexity of genome.