Initiation of replication at oriC starts with formation of a complex that ultimately requires six proteins: DnaA, DnaB, DnaC, HU, gyrase, and SSB.

TLS Online TPP Program

#Id: 2818

#Unit 13. Methods in Biology

In the HIC some highly hydrophobic proteins may not even be eluted in the total absence of salt in such case water-miscible organic solvent such as propanol or ethylene glycol to the column buffer solution.

TLS Online TPP Program

#Id: 2819

#Unit 13. Methods in Biology

Ion-exchange chromatography relies on the attraction between oppositely charged stationary phase, known as an ion exchanger, and analyte.

TLS Online TPP Program

#Id: 2820

#Unit 13. Methods in Biology

Ion-exchange chromatography is frequently chosen for the separation and purification of proteins, peptides, nucleic acids, polynucleotides and other charged molecules, mainly because of its high resolving power and high capacity.

TLS Online TPP Program

#Id: 2821

#Unit 13. Methods in Biology

In Ion-exchange chromatography There are two types of ion exchanger, namely cation  and anion exchangers.

TLS Online TPP Program

#Id: 2822

#Unit 13. Methods in Biology

In Ion-exchange chromatography Cation exchangers possess negatively charged groups and these will attract positively charged cations & Anion exchangers have positively charged groups that will attract negatively charged anions

TLS Online TPP Program

#Id: 2823

#Unit 13. Methods in Biology

Examples of commonly used ion exchangers