DnaA promotes replication

SeqA delays new round of replication by inhibiting dam methylase binding

Dam methylase provide substrate for DnaA

TLS Online TPP Program

#Id: 6014

#Unit 3. Fundamental Processes

Helicases are loaded during G1 are only activated to unwind DNA and initiate replication after cells pass from the G1 to the S phase of the cell cycle in effect with S phase CDKC

TLS Online TPP Program

#Id: 6015

#Unit 3. Fundamental Processes

The origin enters S phase in the condition of a prereplication complex, which contains ORC, Cdc6, Cdt1, and the inactive helicase

TLS Online TPP Program

#Id: 6016

#Unit 3. Fundamental Processes

The MCM2-7 proteins form a six-member ring-shaped complex around DNA. MCM2,3,5 are regulatory, while MCM4,6,7 have the helicase activity.

TLS Online TPP Program

#Id: 6017

#Unit 3. Fundamental Processes

In mammalian cells, Cdt1 is targeted for degradation by the action of a protein complex that is recruited to the origin of replication by PCNA, the eukaryotic counterpart of the bacterial b clamp.

TLS Online TPP Program

#Id: 6018

#Unit 3. Fundamental Processes

As the transition from G1 to S phase begins, CDK/cyclins recruit cdc45 and the GINS complex to the MCM helicase, which then becomes known as the CMG complex (Cdc45-MCM-GINS)

TLS Online TPP Program

#Id: 6019

#Unit 3. Fundamental Processes