Potassium channels remain open for another few tenths of a second, with resultant excess negativity inside the fiber; this process is called hyperpolarization.

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#Id: 2509

#Unit 13. Methods in Biology

The most commonly used general protein stain for detecting protein on gels is the sulfated trimethylamine dye Coomassie Brilliant Blue R-250 (CBB).

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#Id: 2510

#Unit 13. Methods in Biology

Silver staining gives higher sensitivity comparison to Coomassie Brilliant Blue (CBB).

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#Id: 2511

#Unit 13. Methods in Biology

Glycoproteins have traditionally been detected on protein gels by use of the periodic acid–Schiff ( PAS) stain

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#Id: 2512

#Unit 13. Methods in Biology

Quantitative analysis (i.e., measurements of the relative amounts of different proteins in a sample) can be achieved by scanning densitometry .

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#Id: 2513

#Unit 13. Methods in Biology

Larger fragment DNA are isolated by using agarose gel while shorter fragment are separated by 3.5% polyacrylamide gels are used to separate DNA in the range 80–1000 nt and 12% gels to resolve fragments of between 20 and 100 nt.

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#Id: 2514

#Unit 13. Methods in Biology

Pulsed-Field Gel Electrophoresis are used to separate very large fragment of DNA 2 × 103 kb, Essentially, this allows the separation of whole chromosomes by electrophoresis.