The slow return of the K + channels to the closed state also explains the after-hyperpolarization, followed by a return to the resting membrane potential

TLS Online TPP Program

#Id: 2848

#Unit 13. Methods in Biology

SEC chromatographic technique for the separation of molecules on the basis of their molecular size and shape exploits the molecular sieve properties of a variety of porous materials.

TLS Online TPP Program

#Id: 2849

#Unit 13. Methods in Biology

SEC a column of microparticulate cross-linked copolymers generally of either styrene or divinylbenzene and with a narrow range of pore sizes is in equilibrium with a suitable mobile phase for the analytes to be separated.

TLS Online TPP Program

#Id: 2850

#Unit 13. Methods in Biology

In SEC if the analyte is large and completely excluded from the mobile phase within the particle, Kd = 0, whereas, if the analyte is sufficiently small to gain complete access to the inner mobile phase, Kd = 1 where Kd is distribution coefficient.

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#Id: 2851

#Unit 13. Methods in Biology

In Size exclusion chromatography (SEC) Separation of different size molecules by exclusion chromatography. Large, excluded molecules are eluted first in the void.

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#Id: 2852

#Unit 13. Methods in Biology

Stationary phases commonly used for exclusion chromatography

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#Id: 2853

#Unit 13. Methods in Biology

Molecular (sub-)structures responsible for interaction with electromagnetic radiation are called chromophores.