Mutants that cannot be expressed at all are called uninducible. Mutants that are continuously expressed are called constitutive mutants.

TLS Online TPP Program

#Id: 2815

#Unit 13. Methods in Biology

HIC column is made up of phenyl-Sepharose, where phenyl groups are bonded to the insoluble support Sepharose.

TLS Online TPP Program

#Id: 2816

#Unit 13. Methods in Biology

When in the protein solution divalent salts, or multivalent salts the protein separated in form of aggregate.

TLS Online TPP Program

#Id: 2817

#Unit 13. Methods in Biology

In the salt fractionation most polar will be fractionated with large amount of salt.

TLS Online TPP Program

#Id: 2818

#Unit 13. Methods in Biology

In the HIC some highly hydrophobic proteins may not even be eluted in the total absence of salt in such case water-miscible organic solvent such as propanol or ethylene glycol to the column buffer solution.

TLS Online TPP Program

#Id: 2819

#Unit 13. Methods in Biology

Ion-exchange chromatography relies on the attraction between oppositely charged stationary phase, known as an ion exchanger, and analyte.

TLS Online TPP Program

#Id: 2820

#Unit 13. Methods in Biology

Ion-exchange chromatography is frequently chosen for the separation and purification of proteins, peptides, nucleic acids, polynucleotides and other charged molecules, mainly because of its high resolving power and high capacity.