Cdc6 has an ATPase activity that is required for it to support initiation.

TLS Online TPP Program

#Id: 2287

#Unit 3. Fundamental Processes

How tightly does core enzyme v. holoenzyme bind DNA?

TLS Online TPP Program

#Id: 2288

#Unit 3. Fundamental Processes

The effect of temperature on the dissociation of the polymerase–T7 DNA complex. 

TLS Online TPP Program

#Id: 2289

#Unit 3. Fundamental Processes

higher temperature favors tighter binding between RNA polymerase holoenzyme and T7 DNA

TLS Online TPP Program

#Id: 2290

#Unit 3. Fundamental Processes

RNA polymerase holoenzyme binds DNA loosely at first

a. Binds at promoter initially

b. Scans along the DNA until it finds one

TLS Online TPP Program

#Id: 2291

#Unit 3. Fundamental Processes

Isomerization, does not require energy derived from ATP hydrolysis and is instead the result of a spontaneous conformational change in the DNA– enzyme complex to a more energetically favorable form. 

TLS Online TPP Program

#Id: 2292

#Unit 3. Fundamental Processes

Isomerization is essentially irreversible and once complete, typically guarantees that transcription will subsequently initiate .