TLS Online TPP Program

#Id: 427

#Unit 2. Cellular Organization

Chromatin contains DNA, Histones, Nonhistone Proteins and RNA. RNA polymerase is a prominent nonhistone protein

TLS Online TPP Program

#Id: 428

#Unit 2. Cellular Organization

Individual nucleosomes can be obtained by treating chromatin with the endonuclease micrococcal nuclease (MNase), which cuts the DNA between nucleosomes, a region known as linker DNA.

TLS Online TPP Program

#Id: 429

#Unit 2. Cellular Organization

Mononucleosomes typically have 200 bp DNA. End-trimming reduces the length of DNA first to 165 bp and then generates core particles with 146 bp.

TLS Online TPP Program

#Id: 430

#Unit 2. Cellular Organization

Mnase digestion result on centrifugation and electrophoresis

TLS Online TPP Program

#Id: 431

#Unit 2. Cellular Organization

Core DNA has an invariant length of 146 bp the minimum length of DNA needed to form a stable monomeric nucleosome, and is relatively resistant to digestion by nucleases.

TLS Online TPP Program

#Id: 432

#Unit 2. Cellular Organization

Core DNA invariant length of 146 bp contains core and linker histones so Mnase cuts after H1 binding site.