IPTG (isopropyl b-D-1-thiogalactopyranoside) 

X-gal (5-bromo4-chloro-3-indolyl-b-D-galactopyranoside). When acted on by that enzyme, X-gal releases a blue color that makes it useful as substrate in assays.

TLS Online TPP Program

#Id: 3592

#Unit 3. Fundamental Processes

A codon is a triplet of nucleotides that codes for a specific amino acid.

TLS Online TPP Program

#Id: 3593

#Unit 3. Fundamental Processes

The side chains of amino acids have little or no specific affinity for the purine and pyrimidine bases found in RNA.

TLS Online TPP Program

#Id: 3594

#Unit 3. Fundamental Processes

In 1961 Marshall Nirenberg and Heinrich Matthaei reported the first breakthrough in reading nucleotide codon in amino acid frame.

TLS Online TPP Program

#Id: 3596

#Unit 3. Fundamental Processes

Nirenberg and Matthaei therefore concluded that the triplet codon UUU encodes phenylalanine, poly(C) proline (polyproline), polyadenylate, poly(A), encodes polylysine.

TLS Online TPP Program

#Id: 3597

#Unit 3. Fundamental Processes

“Dictionary” of amino acid code words in mRNAs.

TLS Online TPP Program

#Id: 3599

#Unit 3. Fundamental Processes

The third base of each codon plays a lesser role in specifying an amino acid than the first two.