IPTG (isopropyl b-D-1-thiogalactopyranoside) 

X-gal (5-bromo4-chloro-3-indolyl-b-D-galactopyranoside). When acted on by that enzyme, X-gal releases a blue color that makes it useful as substrate in assays.

TLS Online TPP Program

#Id: 1312

#Unit 2. Cellular Organization

Cardiac glycosides (e.g., ouabain and digitalis) are a class of drugs that inhibits Na+-K+ ATPase.

TLS Online TPP Program

#Id: 1504

#Unit 2. Cellular Organization

Microtubules are polymers of the protein tubulin.

TLS Online TPP Program

#Id: 1505

#Unit 2. Cellular Organization

The tubulin subunit is a heterodimer formed from two closely related globular proteins called a-tubulin and b-tubulin.

TLS Online TPP Program

#Id: 1506

#Unit 2. Cellular Organization

a-tubulin and b-tubulin comprising 445–450 amino acids, which are tightly held together by noncovalent bonds.

TLS Online TPP Program

#Id: 1507

#Unit 2. Cellular Organization

Each α or β monomer has a binding site for one molecule of GTP.

TLS Online TPP Program

#Id: 1508

#Unit 2. Cellular Organization

The GTP that is bound to α-tubulin is physically trapped at the dimer interface and is never hydrolyzed.