binding by a ribosome, many prokaryotic ORFs contain a short sequence upstream (on the 5’ side) of the start codon called the ribosome binding site (RBS). This element is also referred to as a polypurine stretch Shine–Dalgarno sequence.

TLS Online TPP Program

#Id: 817

#Unit 3. Fundamental Processes

Region 2.3, 2.4, and 4.2 contact bases in the -10 and -35 elements, respectively; 

sigma factor region 1.2 contacts the promoter region just downstream from the -10 element, and region 3.0 contacts the promoter region just upstream from the -10 element.

 

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#Id: 818

#Unit 3. Fundamental Processes

Sigma factor Region 2.3 is responsible for melting the DNA at -10 element.

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#Id: 1841

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 1842

#Unit 3. Fundamental Processes

Eukaryotic nuclei contain RNA polymerase I, RNA polymerase II, and RNA polymerase III can be separated by using DEAE-Sephadex ion-exchange chromatography

TLS Online TPP Program

#Id: 1843

#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits, Rpb1 and Rpb2

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#Id: 1844

#Unit 3. Fundamental Processes

Rpo I =14 subunits

Rpo II = 12 subunits

Rpo III = 17 subunits

There are five common subunits

Rpb5, Rpb6, Rpb8, Rpb10, Rpb12