TLS Online TPP Program

#Id: 2520

#Unit 13. Methods in Biology

Capillary zone electrophoresis can analyze hybridization probes, gene-cloning, DNA sequencing, polymerase chain reaction (PCR), HIV load, site-directed mutagenesis, & antisense therapeutics.

TLS Online TPP Program

#Id: 2521

#Unit 13. Methods in Biology

Point mutations in DNA, quantification of DNA, Chiral compounds all ca be analyzed by using Capillary zone electrophoresis.

TLS Online TPP Program

#Id: 2522

#Unit 13. Methods in Biology

Output of capillary zone electrophoresis known as electropherogram.

TLS Online TPP Program

#Id: 2523

#Unit 13. Methods in Biology

Microchip electrophoresis is also based on free flow.

TLS Online TPP Program

#Id: 2524

#Unit 13. Methods in Biology

Slab gels can develop a “Smiling Effect” When in a gel more Joule heat is produced than can be dissipated, leads to the higher mobility of the ions in the center, resulting in an uneven distribution of the zones, These zones bend up at the two lateral sides, causing “smiling.” 

TLS Online TPP Program

#Id: 2525

#Unit 13. Methods in Biology

The PCR consists of three defined sets of times and temperatures, termed steps

denaturatio

Annealing 

extension