Antisense morpholinos to eliminate three BMP antagonists (Noggin, Chordin, and Follistatin) in Xenopus. The resulting embryos had catastrophic failure of dorsal development and lacked neural plates and dorsal mesoderm

TLS Online TPP Program

#Id: 2905

#Unit 3. Fundamental Processes

DnaA then acts at three A-T-rich 13 -bp tandem repeats located on the left side of oriC

TLS Online TPP Program

#Id: 2906

#Unit 3. Fundamental Processes

Two to four monomers of Dna AA TP bind at the origin, and after release of DiaA, they recruit two "pre-priming" complexes of the DnaB helicase bound to DnaC-ATP, so that there is one DnaB-DnaC-ATP complex for each of the two (bidirectional) replication forks.

TLS Online TPP Program

#Id: 2907

#Unit 3. Fundamental Processes

The function of DnaC is that of a chaperone to repress the helicase activity of DnaB until it is needed.

TLS Online TPP Program

#Id: 2908

#Unit 3. Fundamental Processes

Each DnaB-DnaC complex consists of six DnaC monomers bound to a hexamer of DnaB

TLS Online TPP Program

#Id: 2909

#Unit 3. Fundamental Processes

he prepriming complex generates a protein aggregate of 480 kD, which corresponds to a sphere of radius 6 nm

TLS Online TPP Program

#Id: 2912

#Unit 3. Fundamental Processes

Each DnaB activates a DnaG primase-in one case to initiate the leading strand, and in the other to initiate the first Okazaki fragment of the lagging strand