A posterior-to-anterior gradient of Nanos protein A posterior-to-anterior gradient of Caudal protein

TLS Online TPP Program

#Id: 568

#Unit 2. Cellular Organization

Leaky Expression

In repressed state, an occasional transcript gets made. This happens because every so often, RNA polymerase will manage to bind the promoter in place of the Lac repressor. This leakiness encode meager level of Z, Y and A.

TLS Online TPP Program

#Id: 569

#Unit 2. Cellular Organization

Leaky Expression cannot happen in P- and Z- mutants

TLS Online TPP Program

#Id: 570

#Unit 2. Cellular Organization

The lac operator is 21-bp sequence with twofold symmetric and is recognized by two subunits of Lac repressor

 

TLS Online TPP Program

#Id: 571

#Unit 2. Cellular Organization

Constitutive mutations occur at 8 positions in the operator between +5 and +17 called OC

TLS Online TPP Program

#Id: 572

#Unit 2. Cellular Organization

Repressor binds with O1and O3 tonhibit expression of lacZ

TLS Online TPP Program

#Id: 573

#Unit 2. Cellular Organization

Effects of mutations in the three lac operators

All three operators are essential for effective repression.