Eight approximately 100-nm long filaments extend into the nucleoplasm with the distal ends of these filaments joined by a terminal ring, forming a structure called the nuclear basket.

TLS Online TPP Program

#Id: 4477

#Unit 13. Methods in Biology

DIC microscopy is used for visualizing extremely small details and thick objects.

TLS Online TPP Program

#Id: 4478

#Unit 13. Methods in Biology

DIC microscopy easily defines the outlines of large organelles, such as the nucleus and vacuole.

TLS Online TPP Program

#Id: 4479

#Unit 13. Methods in Biology

Formaldehyde a common fixative, crosslinks amino groups on adjacent molecules

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#Id: 4480

#Unit 13. Methods in Biology

Histological samples are often stained with hematoxylin and eosin (“H&E stain”).

TLS Online TPP Program

#Id: 4481

#Unit 13. Methods in Biology

Hematoxylin binds to basic amino acids (lysine and arginine).

TLS Online TPP Program

#Id: 4482

#Unit 13. Methods in Biology

Eosin binds to acidic molecules (DNA and side chains of aspartate and glutamate).