Sigma inhibits non specific binding of holoenzyme so fourth slides rapidly over non promoters sequences but binding strongly to promoters

TLS Online TPP Program

#Id: 2991

#Unit 13. Methods in Biology

Isopycnic separation, centrifugation is continued until the desired particles have
reached their isopycnic position in the liquid density gradient.

TLS Online TPP Program

#Id: 2995

#Unit 13. Methods in Biology

Subcellular membrane systems that can be isolated by differential centrifugation.

TLS Online TPP Program

#Id: 2999

#Unit 13. Methods in Biology

Flow chart of the fractionation protocol of these membranes from tissue homogenates
using differential centrifugation and density gradient methodology.

TLS Online TPP Program

#Id: 3000

#Unit 13. Methods in Biology

Crude differential centrifugation techniques can be conveniently employed to isolate
intact mitochondria and microsomes.

TLS Online TPP Program

#Id: 3001

#Unit 13. Methods in Biology

Caesium chloride is widely used for the banding of DNA and the isolation of
plasmids, nucleoproteins and viruses

TLS Online TPP Program

#Id: 3002

#Unit 13. Methods in Biology

Sodium bromide and sodium iodide are employed for the fractionation of lipoproteins
and the banding of DNA or RNA molecules, respectively.