Elongation phase

short oligomer rapidly increases in length by the addition of actin monomers to both of its ends, causing filament elongation

TLS Online TPP Program

#Id: 2855

#Unit 13. Methods in Biology

The presence of several conjugated double bonds in organic molecules possess  p ®p* transitions in the UV/Vis range of the electromagnetic spectrum. Such molecules are very useful tools in colorimetric applications.

TLS Online TPP Program

#Id: 2856

#Unit 13. Methods in Biology

Common colorimetric and UV absorption assays

TLS Online TPP Program

#Id: 2857

#Unit 13. Methods in Biology

Concentration of protein can be analyzed by to method constructive and destructive method .

TLS Online TPP Program

#Id: 2858

#Unit 13. Methods in Biology

Destructive method uses Folin reagent, Biuret, BCA, Coomassie Brilliant Blue and give absorbance at 660, 540, 562, 595 nm of wavelength respectively.

TLS Online TPP Program

#Id: 2859

#Unit 13. Methods in Biology

The constructive method involved direct analysis of protein and gives absorbance at wavelength of 280 nm.

TLS Online TPP Program

#Id: 2860

#Unit 13. Methods in Biology

Carotenoids gives absorbance between 400 – 500 nm of wavelength while porphyrin gives absorbance at 400 nm of wavelength known as Soret band.