The PCR consists of three defined sets of times and temperatures, termed steps

a) Denaturation at 94°C, for 0.5 min

b) Primer annealing at 55°C for 1.5 min

c) Extension at 72°C for 1 min

TLS Online TPP Program

#Id: 2871

#Unit 13. Methods in Biology

The ratio T= I/I0 is called transmission.

TLS Online TPP Program

#Id: 2872

#Unit 13. Methods in Biology

Algebraic transformation of the exponential correlation into an expression based on the decadic logarithm yields the law of Beer–Lambert:

where [d] = 1cm, [c] = 1moldm-3, and [e] = 1dm3mol-1 cm1. e is the molar absorption   coefficient.

where [d] = 1cm, [c] = 1moldm-3, and [e] = 1dm3mol-1 cm1. e is the molar absorption coefficient.

TLS Online TPP Program

#Id: 2873

#Unit 13. Methods in Biology

Absorbance of the sample is displayed on the spectrophotometer.

TLS Online TPP Program

#Id: 2874

#Unit 13. Methods in Biology

The Beer–Lambert law is valid for low concentrations only.

TLS Online TPP Program

#Id: 2875

#Unit 13. Methods in Biology

According to the Beer–Lambert law, absorbance is linearly proportional to the concentration of chromophores.

TLS Online TPP Program

#Id: 2876

#Unit 13. Methods in Biology

Beer–Lambert law might not be the case any more in samples with high absorbance.