Regeneration of the CO2 acceptor ribulose 1,5-bisphosphate. 

The cycle is completed by regeneration of ribulose 1,5-bisphosphate through a series of ten enzyme-catalyzed reactions, one requiring ATP.

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#Unit 3. Fundamental Processes

The E. coli enzyme acts by antagonizing the replication helicase in a direction a) manner by direct contact between the DnaB helicase and Tus.

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#Unit 3. Fundamental Processes

Deletion of the ter sites does not, however, prevent normal replication cycles from occurring, although it does affect segregation of the daughter chromosomes.

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#Unit 3. Fundamental Processes

The two replication forks meet and halt in a region approximately halfway around the chromosome from the origin.

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#Unit 3. Fundamental Processes

 In E. coli, two clusters of five ter sites each, including terk, -I, -E, -D, and -A on one side and terC, -B, -F, -G, and -H on the other, are located -100 kb on either side of this termination region.

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#Unit 3. Fundamental Processes

Each set of ter sites allows a replication fork into the termination region, but does not allow it out the other side.For example, replication fork 1 can pass through terC and terB into the region, but it cannot continue past terE, -D, and -A. This arrangement creates a "replication fork trap."

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#Unit 3. Fundamental Processes

 mtDNA replication and segregation to daughter mitochondria is stochastic.