TLS Online TPP Program

#Id: 886

#Unit 13. Methods in Biology

The most common STRs in humans are A-rich units: A, AC, AAAN, AAN, and AG. The STR locus is named as, for example, D3S1266, where D represents DNA, 3 means chromosome 3 on which the STR locus locates, S stands for STR, and 1266 is the unique identifier.

TLS Online TPP Program

#Id: 887

#Unit 13. Methods in Biology

Repeat expansion diseases are analysed by PCR easily.

TLS Online TPP Program

#Id: 888

#Unit 13. Methods in Biology

The repeat region of the Huntington disease locus (HD) in six individuals is amplified by PCR and analyzed by gel electrophoresis as shown in the following figure; the numbers to the right indicate the sizes of the PCR products in bp.

               

TLS Online TPP Program

#Id: 889

#Unit 13. Methods in Biology

A recognition site frequency every 44 (256) bp, 46 (4096) bp and 48 (65 536) bp, if A=T=G=C.

TLS Online TPP Program

#Id: 890

#Unit 13. Methods in Biology

Frequency of recognition site depends on A/T::G/C ratio of genome.

TLS Online TPP Program

#Id: 891

#Unit 13. Methods in Biology

The nuclear genome of mammals is 40% G+C and the dinucleotide CG is fivefold less common than statistically expected.