Terminate the visual response involves phosphorylation of activated (R*) rhodopsin, but not its dark (R) form

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#Id: 2804

#Unit 13. Methods in Biology

In NPLC solvents form an elutropic series based on their polarity. Such a series in

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#Id: 2805

#Unit 13. Methods in Biology

 In NPLC order of elution of analytes is such that the least polar is eluted first and the most polar last.

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#Id: 2806

#Unit 13. Methods in Biology

The main applications of normal-phase liquid chromatography are its use to separate

analytes that have low water solubility and those that are not amenable to reversed phase liquid chromatography.

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#Id: 2807

#Unit 13. Methods in Biology

The pH of the buffer selected as eluent should be at least one pH unit above or below the isoionic point of the analytes.

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#Id: 2808

#Unit 13. Methods in Biology

Reversed-phase liquid chromatography form of liquid chromatography, which has many similarities with hydrophobic interaction chromatography (HIC).

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#Id: 2809

#Unit 13. Methods in Biology

In RPLC the stationary phase is non-polar and the mobile phase relatively polar, hence the name reversed-phase