Nurturing Life Sciences
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#Unit 13. Methods in Biology
The MTT assay can measure cell proliferation or cell death.
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bromodeoxyuridine (BrdU) is rapidly phosphorylated and incorporated in its place into newly synthesized DNA hence BrdU is used to detect replicating cells
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If cells that have incorporated a high level of BrdU are exposed to light, they will photolyse; this has been used to selectively kill newly dividing cells.
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Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle.