In the dark, the maximum absorption of the FMN chromophore is around 450 nm. 

Blue light induces the formation of a covalent bond between FMN and a cysteine residue, shifting the absorption maximum to 390 nm via a LOV660 intermediate form. The reaction is reversible in darkness.

TLS Online TPP Program

#Id: 2803

#Unit 13. Methods in Biology

In NPLC the most popular stationary phase is an alkylamine bonded to silica. The mobile phase is generally an organic solvent such as hexane, heptane, dichloromethane or ethyl acetate.

TLS Online TPP Program

#Id: 2819

#Unit 13. Methods in Biology

Ion-exchange chromatography relies on the attraction between oppositely charged stationary phase, known as an ion exchanger, and analyte.

TLS Online TPP Program

#Id: 769

#Unit 3. Fundamental Processes

After release of the abortive product, the enzyme again begins synthesizing RNA at position +1 called as Initiation

TLS Online TPP Program

#Id: 6379

#Unit 2. Cellular Organization

Leaky Expression

In repressed state, an occasional transcript gets made. This happens because every so often, RNA polymerase will manage to bind the promoter in place of the Lac repressor. This leakiness encode meager level of Z, Y and A.

TLS Online TPP Program

#Id: 9073

#Unit 4. Cell Communication and Cell Signaling