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#Question id: 19888
#Unit 14. Methods in Biology
There are some typical enzyme used in the manipulation of nucleic acid;
|
ENZYMES |
USE IN
NUCLEIC ACID MANIPULATION |
|
A) klenow |
i)
DNA-dependent RNA polymerase |
|
B) T4 DNA
polymerase |
ii)
Single-strand specific nuclease |
|
C) Taq DNA
polymerase |
iii) DNA pol
I lacks 5’→ 3’ exonuclease activity |
|
D) T7 RNA
polymerase |
iv)
Thermostable DNA polymerase used in PCR |
|
E) S1
nuclease |
v) Lack 5’→ 3’ exonuclease activity |