Column A (Method)	Column B (Material to Sterilize)
1. Surface Sterilization	A. Heat labile growth regulators (GA, Zeatin)
2. Filter Sterilization	B. Glassware and Teflon plastic ware
3. Dry Heat	C. Plant materials (explants, ovules)
4. Wiping with 70% Ethanol	D. Hands of operator and cabinet platform

TLS Online TPP Program

#Question id: 39292

#Plant Biotechnology

Q. Assertion: Symmetric hybrids allow for the transfer of genes between species that are sexually incompatible. 

Reason: Somatic hybridization bypasses the requirements of pollination and fertilization, enabling fusion between even completely sterile lines like monoploids.

TLS Online TPP Program

#Question id: 39293

#Plant Biotechnology

Q. Assertion: Cybridization enables the creation of unique organelle combinations that cannot be produced through sexual reproduction. 

Reason: Somatic fusion allows the mitochondria of one species to be combined with the chloroplasts of another, often resulting in recombinant organellar genomes. 

TLS Online TPP Program

#Question id: 39294

#Plant Biotechnology

Q. Assertion: Somatic hybridization is currently the primary method used for the commercial improvement of all major cereal and pulse crops. 

Reason: Techniques for protoplast isolation, culture, and fusion are not yet readily available for many important cereals and pulses.

TLS Online TPP Program

#Question id: 39295

#Plant Biotechnology

Q. Assertion: Chromosome elimination in somatic hybrids is a highly predictable process used for precise gene mapping. 

Reason: Genetic instability is a common limitation in somatic hybrids, particularly in wide interfamilial crosses, often resulting in abnormal or sterile plants.

TLS Online TPP Program

#Question id: 39296

#Plant Biotechnology

Q. Which parental combination was used to develop high nicotine content in tobacco?

TLS Online TPP Program

#Question id: 39297

#Plant Biotechnology

Q. Which of the following hybrids is associated with low erucic acid content?