Column A	Column B
1. HEPA Filter	A. Used for heat-labile vitamins & enzymes
2. Autoclave	B. Filters out particles > 0.3 µm
3. Membrane Filter (0.45 μm)	C. Sterilizes culture vessels using steam pressure
4. HgCl₂ (Mercuric Chloride)	D. Agent used for surface sterilization of explants

TLS Online TPP Program

#Question id: 12933

#Bio-Informatics

Choose the correct order for the cloning process.

1. The production of cDNA is carried out using an enzyme termed Reverse transcriptase.

2. mRNA cannot be used directly in cloning.

3. It is possible to synthesis complementary DNA molecule to all the mRNA from the selected tissue.

4. The cDNA may be inserted into vectors and then cloned.

TLS Online TPP Program

#Question id: 12934

#Bio-Informatics

Reverse transcriptase enzyme is isolated from____

TLS Online TPP Program

#Question id: 12938

#Bio-Informatics

What is the main advantage of using Yeast Artificial Chromosome based vector over other vectors?

1. It has ability to clone very large fragments of DNA.

2. YAC vectors do not contain origin of replication sites 

3. It has insert  range up to 2000 kb.

4. It help in genome mapping and sequencing projects.

TLS Online TPP Program

#Question id: 13032

#Bio-Informatics

In SDS-PAGE, protein sample is first treated with detergent sodium dodecyl sulfate (SDS), in order to

TLS Online TPP Program

#Question id: 13035

#Bio-Informatics

Each band on the gel represents a different protein (or protein subunit); _____ proteins move through the gel more _____ than_____ proteins and therefore are found nearer the _____ of the gel.

TLS Online TPP Program

#Question id: 13036

#Bio-Informatics

In isoelectric focusing the protein will move until