Match the term with the correct definition regarding cell regeneration pathways:

List I (Process)	List II (Description)
A. Organogenesis	1. Direct formation of embryos from somatic cells
B. Somatic Embryogenesis	2. Dedifferentiation → callus → redifferentiation → organs
C. Proliferative Cycle	3. Daughter cells are copies of mother, differentiate without division
D. Quantal Cycle	4. Daughter cells differ from mother, less likely to differentiate

TLS Online TPP Program

#Question id: 12739

#Bio-Informatics

Method used for gene editing and for the development in Gene modifications is known as_______.

TLS Online TPP Program

#Question id: 12911

#Bio-Informatics

Match the following:

A. DNA polymerases              i. Alkaline phosphatase        a.RNA-dependent DNA polymerase, used in cDNA synthesis

B. Reverse transcriptase        ii. T4 DNA ligase                    b. Thermostable DNA polymerase used in PCR

C. Ligases                                 iii.  AMV-RT                            c. Removes 5′-phosphates from DNA and RNA

D. Phosphatases                     iv. Taq DNA pol                      d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond

TLS Online TPP Program

#Question id: 12912

#Bio-Informatics

Name the enzyme which separates the double-helical DNA into single strands.

TLS Online TPP Program

#Question id: 12915

#Bio-Informatics

Which of the following statement is not correct about PCR.

TLS Online TPP Program

#Question id: 12916

#Bio-Informatics

Which statement is correct for Zoo blotting?

TLS Online TPP Program

#Question id: 12917

#Bio-Informatics

Arrange the steps of stages in PCR in correct order.

1. The region to be specifically amplified is made accessible within the complex DNA.

2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.

3. A thermal cycler is used to incrementally decrease the annealing temperature.

4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.