TLS Online TPP Program

#Question id: 37926


Arrange the following steps involved in enzymatic isolation, culture, and regeneration of plant protoplasts in the correct sequence:
A. Enzymatic digestion of cell wall using cellulase and pectinase
 B. Culturing of isolated protoplasts by hanging drop / feeder layer / agar methods
 C. Surface sterilization and epidermis removal from leaf explant
 D. Density gradient centrifugation to separate viable protoplasts
 E. Callus formation followed by regeneration of a complete plant

Choose the correct order:

#Plant Biotechnology
  1. C → A → D → B → E
  2. C → D → A → B → E
  3. A → C → D → B → E
  4. C → A → B → D → E