What will be the resolution of a compound microscope where numerical aperture given is 1.5 at 240 wavelength of light?

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#Question id: 12911

#Applied Microbiology

Match the following:

A. DNA polymerases              i. Alkaline phosphatase        a.RNA-dependent DNA polymerase, used in cDNA synthesis

B. Reverse transcriptase        ii. T4 DNA ligase                    b. Thermostable DNA polymerase used in PCR

C. Ligases                                 iii.  AMV-RT                            c. Removes 5′-phosphates from DNA and RNA

D. Phosphatases                     iv. Taq DNA pol                      d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond

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#Question id: 12912

#Applied Microbiology

Name the enzyme which separates the double-helical DNA into single strands.

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#Question id: 12925

#Applied Microbiology

Another name of TaqMan assay is ____

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#Question id: 12932

#Applied Microbiology

Why mRNA cannot be used directly in cloning?

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#Question id: 12933

#Applied Microbiology

Choose the correct order for the cloning process.

1. The production of cDNA is carried out using an enzyme termed Reverse transcriptase.

2. mRNA cannot be used directly in cloning.

3. It is possible to synthesis complementary DNA molecule to all the mRNA from the selected tissue.

4. The cDNA may be inserted into vectors and then cloned.

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#Question id: 12934

#Applied Microbiology

Reverse transcriptase enzyme is isolated from____