A chemical is said to be fluorescent if it absorbs light at one wavelength (excitation) and emits light (fluoresces) at a specific 

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#Question id: 23157

#Zoology

Immunofluorescence microscopy, the most widely used method of detecting 

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#Question id: 23158

#Zoology

You have purified protein X and injected into an animal so that over a period of weeks, the animal will mount an immune response and make antibodies to protein X. By which technique these generated antibodies can be extracted from blood serum.

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#Question id: 23168

#Zoology

What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?

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#Question id: 23189

#Zoology

Which microscopy is good for live cell imaging?

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#Question id: 23190

#Zoology

If we want to view live cells which microscopy can be best used for this?

1. Structured illumination microscopy

2. Phase contrast microscopy

3. Scanning electron microscopy

4. Cryoelectron microscopy

Which of the following option has correct combination?

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#Question id: 23193

#Zoology

When you try to image two fluorescent spots separated by 75 nm the fluorescence overlap so much that they look like one spot. Which microscopy can resolve this?