TLS Online TPP Program

#Question id: 13032


In SDS-PAGE, protein sample is first treated with detergent sodium dodecyl sulfate (SDS), in order to

#Bio-Informatics
  1. Make the protein become negatively charged.
  2. Make the protein become positively charged.
  3. Renature the protein.
  4. Adjust the pH of protein.
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TLS Online TPP Program

#Question id: 37963

#Plant Biotechnology

In cereal species like rice, wheat, rye, and maize, how can the formation of embryos or calli be specifically induced? 

TLS Online TPP Program

#Question id: 37964

#Plant Biotechnology

Assertion (A): The suppression of 'late' pollen maturation genes is a critical step for successful androgenesis. 
Reason (R): The expression of these 'late' genes promotes the normal gametophytic pathway, preventing the switch to embryogenesis.

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TLS Online TPP Program

#Question id: 37965

#Plant Biotechnology

Assertion (A): During the induction phase of pollen culture, total protein synthesis is completely halted. 
Reason (R): Existing proteins are suppressed, and new proteins like napin (in Brassica napus) are synthesized.

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TLS Online TPP Program

#Question id: 37966

#Plant Biotechnology

Assertion (A): Commitment to androgenesis is achieved primarily by the abolition of pollen maturation gene expression. 
Reason (R): During induction, several hundred genes are upregulated or downregulated to facilitate the developmental switch

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TLS Online TPP Program

#Question id: 37967

#Plant Biotechnology

Assertion (A): Responsive pollen in culture always develops directly into a globular embryo. 
Reason (R): The developmental fate (embryo vs. callus) depends on the plant species.

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TLS Online TPP Program

#Question id: 37968

#Plant Biotechnology

Assertion (A): In cereals like rice and wheat, the choice between embryo or callus formation can be manipulated. 
Reason (R): Modifying the medium composition in these species can induce either embryos or calli.

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