TLS Online TPP Program
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TLS Online TPP Program
#Question id: 38768
#Plant Biotechnology
Q. Statement I: PEG is negatively charged and can interact with cations such as Ca²⁺.
Statement II: Ca²⁺ ions can bind to negatively charged molecules on the plasma membrane, promoting membrane proximity.
TLS Online TPP Program
#Question id: 38769
#Plant Biotechnology
Q. Statement I: During the washing step, PEG may remove plasma membrane components bound to it.
Statement II: Removal of these components can destabilize membrane integrity and promote fusion of adjacent protoplasts.
TLS Online TPP Program
#Question id: 38770
#Plant Biotechnology
Q. Statement I: PEG-induced fusion is a consequence of controlled membrane damage and reorganization.
Statement II: Plasma membrane destabilization increases the likelihood of fusion between closely placed protoplasts.
TLS Online TPP Program
#Question id: 38771
#Plant Biotechnology
Q. Statement I: PEG can interact with both negatively and positively charged components of the plasma membrane.
Statement II: Such interactions reduce electrostatic repulsion between protoplasts.
TLS Online TPP Program
#Question id: 38772
#Plant Biotechnology
Q. Statement I: Protoplasts are isolated by enzymatic digestion of the cell wall using a combination of cellulase and pectinase.
Statement II: Cellulose microfibrils and pectic substances are major structural components of plant cell walls.
TLS Online TPP Program
#Question id: 38773
#Plant Biotechnology
Q.Statement I: The pH of the enzyme solution for protoplast isolation is maintained between 4.7 and 8.0.
Statement II: Enzymes involved in cell wall degradation show activity only at neutral pH.