TLS Online TPP Program
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TLS Online TPP Program
#Question id: 37836
#Plant Biotechnology
| Column A | Column B |
| 1. Dry Heat | A. 121 °C at 15 PSI for 15–20 min |
| 2. Autoclaving | B. 0.45 μm pore size membrane |
| 3. Filter Sterilization | C. 160–180°C for 3 hours |
| 4. Laminar Air Flow | D. HEPA filter (removes particles > 0.3 μm) |
TLS Online TPP Program
#Question id: 37837
#Plant Biotechnology
| Column A (Method) | Column B (Material to Sterilize) |
| 1. Surface Sterilization | A. Heat labile growth regulators (GA, Zeatin) |
| 2. Filter Sterilization | B. Glassware and Teflon plastic ware |
| 3. Dry Heat | C. Plant materials (explants, ovules) |
| 4. Wiping with 70% Ethanol | D. Hands of operator and cabinet platform |
TLS Online TPP Program
#Question id: 37838
#Plant Biotechnology
| Column A (Process) | Column B (Chemical/Agent Used) |
| 1. Surface Sterilization | A. 95% Alcohol (followed by flaming) |
| 2. Flame Sterilization | B. Sodium Hypochlorite / Mercuric Chloride |
| 3. Cleaning Laminar Platform | C. 70% Ethyl Alcohol |
| 4. Air Filtration | D. HEPA (High Efficiency Particulate Air) |
TLS Online TPP Program
#Question id: 37839
#Plant Biotechnology
| Column A | Column B |
| 1. HEPA Filter | A. Used for heat-labile vitamins & enzymes |
| 2. Autoclave | B. Filters out particles > 0.3 µm |
| 3. Membrane Filter (0.45 μm) | C. Sterilizes culture vessels using steam pressure |
| 4. HgCl₂ (Mercuric Chloride) | D. Agent used for surface sterilization of explants |
TLS Online TPP Program
#Question id: 37840
#Plant Biotechnology
In plant tissue culture:
1. High cytokinin: low auxin →
2. High auxin: low cytokinin
TLS Online TPP Program
#Question id: 37841
#Plant Biotechnology
The following statements refer to plant regeneration from explants/tissues:
(A) Cytokinin promotes shoot formation
(B) Auxin promotes root formation
(C) Auxin: Cytokinin ratio determines organ differentiation
(D) Gibberellin is required for both root and shoot formation
Which of the following combinations is correct?