TLS Online TPP Program

#Question id: 15217


Maximum numbers of band achieved in case of 

#Zoology
  1. Uniform gel SDS
  2. Native gel
  3. Gradient gel
  4. All kind of gel produces same number of bands but differs in their sharpening
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TLS Online TPP Program

#Question id: 40411

#Applied Microbiology

Arrange the correct sequence of color changes observed in a Gram-negative bacterium during the Gram staining process at each step:

  1. After Crystal Violet

  2. After Safranin

  3. After Alcohol decolorization

  4. After Iodine treatment

TLS Online TPP Program

#Question id: 40412

#Applied Microbiology

Match the Reagents in List-I with their specific role/function in List-II.

List-I (Reagent)List-II (Function)
(A) Crystal Violet(I) Mordant (Fixes the dye)
(B) Gram's Iodine(II) Counterstain
(C) 95% Ethyl Alcohol(III) Primary Stain
(D) Safranin(IV) Decolorizing Agent

TLS Online TPP Program

#Question id: 40413

#Applied Microbiology

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).

    Assertion (A): After the washing with alcohol (decolorization step), Gram-positive bacteria retain the purple color, while Gram-negative bacteria become colorless.

      Reason (R): Gram-positive bacteria have a thick peptidoglycan layer that traps the Crystal Violet-Iodine complex, whereas Gram-negative bacteria have a high lipid content in their outer membrane which dissolves in alcohol.

      TLS Online TPP Program

      #Question id: 40414

      #Applied Microbiology

      1. Read the following statements regarding the Gram staining technique.
          1. Statement I: Heat fixing is done after adding the crystal violet stain to the slide.
          1. Statement II: Ideally, the iodine solution (mordant) should be kept on the smear for approximately 1 minute.

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      #Question id: 40415

      #Applied Microbiology

      Which of the following errors during the Gram staining procedure would most likely cause a Gram-positive bacterium to appear Gram-negative (pink)?

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      #Question id: 40416

      #Applied Microbiology

      In a eukaryotic cell utilizing the glycerol-3-phosphate shuttle, what is the total yield of ATP via oxidative phosphorylation alone from the complete oxidation of 1 molecule of glucose? (Assume 1 NADH = 2.5 ATP and 1 FADH₂ = 1.5 ATP)