A good vector must have the following properties.

a) It should be able to replicate autonomously.

b) A vector should be ideally less than 10 kb in size

c) The vector should be easy to isolate and purify.

d)  vector should have suitable marker genes that allow easy detection and/or selection of the transformed host cells.

e) it should a multiple cloning site

TLS Online TPP Program

#Question id: 32511

#Biochemistry

Oligomycin inhibits which of the following complexes

TLS Online TPP Program

#Question id: 32513

#Biochemistry

What would you conclude about the mechanism of ATP synthesis:

TLS Online TPP Program

#Question id: 32520

#Biochemistry

Column 1	Column 2
(a) the electron donor	1. NADH
(b) the electron acceptor	2. E-FMN
(c) the conjugate redox pair	3. NAD+/NADH
(d) the reducing agent
(e) the oxidizing agent

Match the following 

TLS Online TPP Program

#Question id: 32521

#Biochemistry

Match the following

Column 1	Column 2
a. Abundant NADH and O2 , but cyanide added	1. all carriers become reduced
b. Abundant NADH, but O2 exhausted	2. all carriers become oxidized.
c. Abundant O2 , but NADH exhausted	3. cytochrome c oxidized.
d. Abundant NADH and O2

TLS Online TPP Program

#Question id: 32521

#Biochemistry

Match the following

Column 1	Column 2
a. Abundant NADH and O2 , but cyanide added	1. all carriers become reduced
b. Abundant NADH, but O2 exhausted	2. all carriers become oxidized.
c. Abundant O2 , but NADH exhausted	3. cytochrome c oxidized.
d. Abundant NADH and O2

TLS Online TPP Program

#Question id: 32524

#Biochemistry

Which of the following sequential reactions correctly represent(s) 

a. NADH dehydrogenase → Ubiquinone → Cytochrome bc1 → Cytochrome c → Cytochrome c oxidase

b. NADH dehydrogenase → Succinate dehydrogenase → Ubiquinone → Cytochrome c → Cytochrome bc1 → Cytochrome c oxidase

c. NADH dehydrogenase → Ubiquinone → Alternative oxidase

d. NADH dehydrogenase → Alternative oxidase → Ubiquinone